Establishment of an indirect ELISA for detection of the novel antifibrotic peptide M10.

ABSTRACT:

Objective

M10 is a ten amino acid peptide generated from the intracellular cytoplasmic tail of the hepatocyte growth factor (HGF) receptor c-Met following cleavage by caspase-3. Recently we reported that M10 interacts with Smad2 and demonstrates antifibrotic properties in vitro and in vivo and can be advanced into a novel antifibrotic remedy. The current study was undertaken to develop an immunoassay to measure M10 concentration in biological specimens.

Experimental design

An Indirect Enzyme-Linked Immunosorbent Assay (ELISA) for detection of M10 in biological fluids was developed using pharmaceutical grade synthetic M10 as a calibrator and commercially available anti-c-Met C12 antibody.

Results

M10 ELISA specifically detected in plasma M10, but not a scrambled peptid

SUBMITTER: Akter T 

PROVIDER: S-EPMC5703532 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature