Rapid Nanopore Sequencing of Plasmids and Resistance Gene Detection in Clinical Isolates.

ABSTRACT: Recent advances in nanopore sequencing technology have led to a substantial increase in throughput and sequence quality. Together, these improvements may permit real-time benchtop genomic sequencing and antimicrobial resistance gene detection in clinical isolates. In this study, we evaluated workflows and turnaround times for a benchtop long-read sequencing approach in the clinical microbiology laboratory using the Oxford Nanopore Technologies MinION sequencer. We performed genomic and plasmid sequencing of three clinical isolates with both MinION and Illumina MiSeq, using different library preparation methods (2D and rapid 1D) with the goal of antimicrobial resistance gene detection. We specifically evaluated the advantages of using plasmid DNA for sequencing and the value of supplementin

SUBMITTER: Lemon JK 

PROVIDER: S-EPMC5703817 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature