The E. coli dicarboxylic acid transporters DauA act as a signal transducer by interacting with the DctA uptake system.

ABSTRACT: The Slc26A/SulP family of ions transporter is ubiquitous and widpsread in all kingdon of life. In E. coli, we have demonstrated that the Slc26 protein DauA is a C₄-dicarboxilic acids (C₄-diC) transporter active at acidic pH. The main C₄-diC transporter active at pH7 is DctA and is induced by C₄-diC via the DcuS/R two component system. DctA interacts with DcuS, the membrane embedded histidine kinase, to transfers DcuS to the responsive state, i.e. in the absence of DctA, DcuS is permanently "on", but its activity is C₄-diC-dependent when in complex with DctA. Using phenotypic characterization, transport assays and protein expression studies, we show that at pH7 full DctA production depends on the presence of DauA. A Bacterial Two Hybrid

SUBMITTER: Karinou E 

PROVIDER: S-EPMC5703999 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature