Novel elvitegravir nanoformulation approach to suppress the viral load in HIV-infected macrophages.

ABSTRACT:

Purpose

Monocytes serve as sanctuary sites for HIV-1 from which virus is difficult to be eliminated. Therefore, an effective viral suppression in monocytes is critical for effective antiretroviral therapy (ART). This study focuses on a new strategy using nanoformulation to optimize the efficacy of ART drugs in HIV-infected monocytes.

Methods

Poly(lactic-co-glycolic acid) (PLGA)-based elvitegravir nanoparticles (PLGA-EVG) were prepared by nano-precipitation technique. The physicochemical properties of PLGA-EVG were characterized using transmission electron microscopy, dynamic light scattering, and Fourier-transform infrared spectroscopy. Cellular uptake study was performed by fluorescence microscopy and flow cytometry. All in vitro experiments were performed by using H

SUBMITTER: Gong Y 

PROVIDER: S-EPMC5704044 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature