Rapid visual detection of binary toxin producing Clostridium difficile by loop-mediated isothermal amplification.

ABSTRACT: The binary toxin Clostridium difficile transferase (CDT) is frequently observed in C. difficile strains and is associated with an increased severity of C. difficile infection. CDT-producing C. difficile infections cause higher fatality rates than infections with CDT negative isolates. Thus, the rapid and accurate identification of a CDT positive C. difficile infection is critical for effective treatment. The present study demonstrates how loop-mediated isothermal amplification (LAMP) can be used to detect CDT-producing C. difficile based on visual observation. This is a low complexity, rapid molecular method that has the potential to be used within a point of care setting. The specificity and sensitivity of the primers in the LAMP reactions for CDT detection were determined using two diffe

SUBMITTER: Yu L 

PROVIDER: S-EPMC5704324 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature