Modulation of the functional association between the HIV-1 intasome and the nucleosome by histone amino-terminal tails.

ABSTRACT:

Background

Stable insertion of the retroviral DNA genome into host chromatin requires the functional association between the intasome (integrase·viral DNA complex) and the nucleosome. The data from the literature suggest that direct protein-protein contacts between integrase and histones may be involved in anchoring the intasome to the nucleosome. Since histone tails are candidates for interactions with the incoming intasomes we have investigated whether they could participate in modulating the nucleosomal integration process.

Results

We show here that histone tails are required for an optimal association between HIV-1 integrase (IN) and the nucleosome for efficient integration. We also demonstrate direct interactions between IN and the amino-terminal tail of human histone H

SUBMITTER: Benleulmi MS 

PROVIDER: S-EPMC5704366 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature