Transient alkalinization of the leaf apoplast stiffens the cell wall during onset of chloride salinity in corn leaves.

ABSTRACT: During chloride salinity, the pH of the leaf apoplast (pH_apo) transiently alkalizes. There is an ongoing debate about the physiological relevance of these stress-induced pH_apo changes. Using proteomic analyses of expanding leaves of corn (Zea mays L.), we show that this transition in pH_apo conveys functionality by (i) adjusting protein abundances and (ii) affecting the rheological properties of the cell wall. pH_apo was monitored in planta via microscopy-based ratio imaging, and the leaf-proteomic response to the transient leaf apoplastic alkalinization was analyzed via ultra-high performance liquid chromatography-MS. This analysis identified 1459 proteins, of which 44 exhibited increased abundance specifically through the chloride-i

SUBMITTER: Geilfus CM 

PROVIDER: S-EPMC5704465 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature