Asparagine and glutamine ladders promote cross-species prion conversion.

ABSTRACT: Prion transmission between species is governed in part by primary sequence similarity between the infectious prion aggregate, PrP^Sc, and the cellular prion protein of the host, PrP^C A puzzling feature of prion formation is that certain PrP^C sequences, such as that of bank vole, can be converted by a remarkably broad array of different mammalian prions, whereas others, such as rabbit, show robust resistance to cross-species prion conversion. To examine the structural determinants that confer susceptibility or resistance to prion conversion, we systematically tested over 40 PrP^C variants of susceptible and resistant PrP^C sequences in a prion conversion assay. Five key residue positions markedly impacted prion conversion, four of which we

SUBMITTER: Kurt TD 

PROVIDER: S-EPMC5704488 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature