HLA genotyping by next-generation sequencing of complementary DNA.

ABSTRACT:

Background

Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem. Although most currently developed technologies use genomic DNA, use of cDNA could enable genotyping with reduced costs in data production and analysis. We thus developed an HLA genotyping system based on next-generation sequencing of cDNA.

Methods

Each HLA gene was divided into 3 or 4 target regions subjected to PCR

SUBMITTER: Segawa H 

PROVIDER: S-EPMC5704545 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature