Robust transcriptional signatures for low-input RNA samples based on relative expression orderings.

ABSTRACT:

Background

It is often difficult to obtain sufficient quantity of RNA molecules for gene expression profiling under many practical situations. Amplification from low-input samples may induce artificial signals.

Results

We compared the expression measurements of low-input mRNA samples, from 25 pg to 1000 pg mRNA, which were amplified and profiled by Smart-seq, DP-seq and CEL-seq techniques using the Illumina HiSeq 2000 platform, with those of the paired high-input (50 ng) mRNA samples. Even with 1000 pg mRNA input, we found that thousands of genes had at least 2 folds-change of expression levels in the low-input samples compared with the corresponding paired high-input samples. Consequently, a transcriptional signature based on quantitative expression values and determined fr

SUBMITTER: Liu H 

PROVIDER: S-EPMC5704640 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature