Developmentally regulated alternative splicing is perturbed in type 1 diabetic skeletal muscle.

ABSTRACT:

Introduction

Type 1 diabetic patients can develop skeletal muscle weakness and atrophy by molecular mechanisms that are not well understood. Alternative splicing (AS) is critical for gene expression in the skeletal muscle, and its dysregulation is implicated in muscle weakness and atrophy. Therefore, we investigated whether AS patterns are affected in type 1 diabetic skeletal muscle contributing to skeletal muscle defects.

Methods

AS patterns were determined by reverse transcription-polymerase chain reaction and levels of RNA binding proteins were assessed by Western blot in type 1 diabetic mouse skeletal muscle and during normal mouse skeletal muscle development.

Results

Five genes with critical functions in the skeletal muscle are misspliced in type 1 diabetic skele

SUBMITTER: Nutter CA 

PROVIDER: S-EPMC5704903 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature