Differential carbonylation of proteins in end-stage human fatty and nonfatty NASH.

ABSTRACT:

Objective

In the liver, a contributing factor in the pathogenesis of non-alcoholic fatty liver disease is oxidative stress leading to the accumulation of highly reactive electrophilic α/β unsaturated aldehydes. The objective of this study was to determine if significant differences were evident when evaluating carbonylation in human end-stage fatty nonalcoholic steatohepatitis (fNASH) compared to end-stage nonfatty NASH (nfNASH).

Methods

Using hepatic tissue obtained from healthy humans and patients diagnosed with end stage nfNASH or fNASH, overall carbonylation was assessed by immunohistochemistry (IHC) and LC-MS/MS followed by bioinformatics.

Results

Picrosirius red staining revealed extensive fibrosis in both fNASH and nfNASH which corresponded with increased react

SUBMITTER: Shearn CT 

PROVIDER: S-EPMC5704928 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature