Characterization of Maternal and Fetal CYP3A-Mediated Progesterone Metabolism.

ABSTRACT:

Introduction

Progesterone is critical for maintaining pregnancy and onset of labor. We evaluated CYP450-mediated progesterone meta-bolism, specifically the contribution of CYP3A isoforms.

Materials and methods

In vitro progesterone metabolism was characterized in human liver microsomes (HLMs) with and without selective cytochrome P450 inhibitors and in recombinant CYP3A4, CYP3A5, and CYP3A7. 6β-hydroxyprogesterone (6β-OHP) and 16α-hydroxyprogesterone (16α-OHP) metabolites were quantified by HPLC/UV and fit to the Michaelis-Menten equation to determine K_m and V_max. The effect of CYP3A5 expression on progesterone clearance was determined by in vitro in vivo extrapolation.

Results

Ketoconazole inhibited formation of both 6β-OHP and 16α-OHP more than

SUBMITTER: Quinney SK 

PROVIDER: S-EPMC5704987 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature