Lifelong haematopoiesis is established by hundreds of precursors throughout mammalian ontogeny.

ABSTRACT: Current dogma asserts that mammalian lifelong blood production is established by a small number of blood progenitors. However, this model is based on assays that require the disruption, transplantation and/or culture of embryonic tissues. Here, we used the sample-to-sample variance of a multicoloured lineage trace reporter to assess the frequency of emerging lifelong blood progenitors while avoiding the disruption, culture or transplantation of embryos. We find that approximately 719 Flk1⁺ mesodermal precursors, 633 VE-cadherin⁺ endothelial precursors and 545 Vav1⁺ nascent blood stem and progenitor cells emerge to establish the haematopoietic system at embryonic days (E)7-E8.5, E8.5-E11.5 and E11.5-E14.5, respectively. We also determined that the spatio-tem

SUBMITTER: Ganuza M 

PROVIDER: S-EPMC5705075 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature