Enhancement of the Intrinsic Peroxidase-Like Activity of Graphitic Carbon Nitride Nanosheets by ssDNAs and Its Application for Detection of Exosomes.

ABSTRACT: The present work investigates the capability of single-stranded DNA (ssDNA) in enhancing the intrinsic peroxidase-like activity of the g-C₃N₄ nanosheets (NSs). We found that ssDNA adsorbed on g-C₃N₄ NSs could improve the catalytic activity of the nanosheets. The maximum reaction rate of the H₂O₂-mediated TMB oxidation catalyzed by the ssDNA-NSs hybrid was at least 4 times faster than that obtained with unmodified NSs. The activity enhancement could be attributed to the strong interaction between TMB and ssDNA mediated by electrostatic attraction and aromatic stacking and by both the length and base composition of the ssDNA. The high catalytic activity of the ssDNA-NSs hybrid permitted sensitive colorimetric detection of exos

SUBMITTER: Wang YM 

PROVIDER: S-EPMC5705088 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature