Polyclonal antibodies for the detection of Trypanosoma cruzi circulating antigens.

ABSTRACT:

Background

Detection of Trypanosoma cruzi antigens in clinical samples is considered an important diagnostic tool for Chagas disease. The production and use of polyclonal antibodies may contribute to an increase in the sensitivity of immunodiagnosis of Chagas disease.

Methodology/principal findings

Polyclonal antibodies were raised in alpacas, rabbits, and hens immunized with trypomastigote excreted-secreted antigen, membrane proteins, trypomastigote lysate antigen and recombinant 1F8 to produce polyclonal antibodies. Western blot analysis was performed to determine specificity of the developed antibodies. An antigen capture ELISA of circulating antigens in serum, plasma and urine samples was developed using IgY polyclonal antibodies against T. cruzi membrane antigens (captu

SUBMITTER: Malaga-Machaca ES 

PROVIDER: S-EPMC5705163 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature