A predictive model of asymmetric morphogenesis from 3D reconstructions of mouse heart looping dynamics.

ABSTRACT: How left-right patterning drives asymmetric morphogenesis is unclear. Here, we have quantified shape changes during mouse heart looping, from 3D reconstructions by HREM. In combination with cell labelling and computer simulations, we propose a novel model of heart looping. Buckling, when the cardiac tube grows between fixed poles, is modulated by the progressive breakdown of the dorsal mesocardium. We have identified sequential left-right asymmetries at the poles, which bias the buckling in opposite directions, thus leading to a helical shape. Our predictive model is useful to explore the parameter space generating shape variations. The role of the dorsal mesocardium was validated in Shh^-/- mutants, which recapitulate heart shape changes expected from a persistent dorsal

SUBMITTER: Le Garrec JF 

PROVIDER: S-EPMC5705212 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature