Genome-Wide Maps of m6A circRNAs Identify Widespread and Cell-Type-Specific Methylation Patterns that Are Distinct from mRNAs.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most abundant internal modification of mRNAs and is implicated in all aspects of post-transcriptional RNA metabolism. However, little is known about m⁶A modifications to circular (circ) RNAs. We developed a computational pipeline (AutoCirc) that, together with depletion of ribosomal RNA and m⁶A immunoprecipitation, defined thousands of m⁶A circRNAs with cell-type-specific expression. The presence of m⁶A circRNAs is corroborated by interaction between circRNAs and YTHDF1/YTHDF2, proteins that read m⁶A sites in mRNAs, and by reduced m⁶A levels upon depletion of METTL3, the m⁶A writer. Despite sharing m⁶A readers and writers, m⁶A circRNAs are

SUBMITTER: Zhou C 

PROVIDER: S-EPMC5705222 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature