Comparison of Methods for Analyzing Human Adipose Tissue Macrophage Content.

ABSTRACT:

Objective

The relationship between inflammation, obesity, and adverse metabolic conditions is associated with adipose tissue macrophages (ATM). This study compared the measurements of human ATM using flow cytometry, immunohistochemistry (IHC), and real-time polymerase chain reaction (RT-PCR) of ATM markers.

Methods

A new software program (AMCounter) was evaluated to help measure ATM using IHC, and this was compared to flow cytometry and RT-PCR.

Results

IHC had good intraindividual reproducibility for total (CD68), proinflammatory (CD14), and anti-inflammatory (CD206) ATM. The AMCounter improved interreader agreement and was more time efficient. Flow cytometry had acceptable intraindividual reproducibility for the percentage of CD68⁺ cells that were CD14

SUBMITTER: Morgan-Bathke M 

PROVIDER: S-EPMC5705319 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature