Fibroblast-fibronectin patterning and network formation in 3D fibrin matrices.

ABSTRACT:

Purpose

We previously reported that fibroblasts migrating within 3-D collagen matrices move independently, whereas fibroblasts within 3-D fibrin matrices form an interconnected network. Similar networks have been identified previously during in vivo corneal wound healing. In this study, we investigate the role of fibronectin in mediating this mechanism of collective cell spreading, migration and patterning.

Methods

To assess cell spreading, corneal fibroblasts were plated within fibrillar collagen or fibrin matrices. To assess migration, compacted cell-populated collagen matrices were nested inside cell-free fibrin matrices. Constructs were cultured in serum-free media containing PDGF, with or without RGD peptide, anti-α5 or anti-fibronectin blocking antibodies. In some expe

SUBMITTER: Miron-Mendoza M 

PROVIDER: S-EPMC5705415 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature