Promoter Specificity and Efficacy in Conditional and Inducible Transgenic Targeting of Lung Macrophages.

ABSTRACT: Conditional and inducible Cre-loxP systems are used to target gene deletion to specific cell lineages and tissues through promoter-restricted expression of the bacterial DNA recombinase, Cre. Although Cre-loxP systems are widely used to target gene deletion in lung macrophages, limited data are published on the specificity and efficiency of "macrophage targeting" Cre lines. Using R26-stop^fl/fl-TdTomato and tetOn-GFP reporter lines, we assessed the specificity and efficiency of four commercially available Cre driver lines that are often considered "macrophage specific." We evaluated two conditional (Csf1r-Cre and LysM-Cre) and two inducible [CX₃CR1-estrogen receptor-Cre (ERCre) and CD68-rtTA] lines. We assessed Cre activation in six resident lung myeloid populations, a

SUBMITTER: McCubbrey AL 

PROVIDER: S-EPMC5705560 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature