Molecular architecture of potassium chloride co-transporter KCC2.

ABSTRACT: KCC2 is a neuron specific K⁺-Cl^- co-transporter that controls neuronal chloride homeostasis, and is critically involved in many neurological diseases including brain trauma, epilepsies, autism and schizophrenia. Despite significant accumulating data on the biology and electrophysiological properties of KCC2, structure-function relationships remain poorly understood. Here we used calixarene detergent to solubilize and purify wild-type non-aggregated and homogenous KCC2. Specific binding of inhibitor compound VU0463271 was demonstrated using surface plasmon resonance (SPR). Mass spectrometry revealed glycosylations and phosphorylations as expected from functional KCC2. We show by electron microscopy (EM) that KCC2 exists as monomers and dimers in solution. Monomers are

SUBMITTER: Agez M 

PROVIDER: S-EPMC5705597 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature