Activation of Erk in ileal epithelial cells engaged in ischemic-injury repair.

ABSTRACT: Intestinal epithelial cells function as a barrier to protect our body from various agents; therefore, any damage to these cells must be immediately repaired. Several in vivo and vitro studies have shown the involvement of Erk (extracellular signal-regulated kinase) in the regeneration process; however, the spatial regulation of Erk related to tissue morphology has not been well documented. Using two-photon microscopy and mice carrying a Förster resonance energy transfer-based biosensor, we here monitored the Erk activity in the ileal epithelial cells of living mice. Forty-eight h after ischemia-induced injury, epithelial cells were observed as a monolayer covering the injured area. The Erk activity in these cells was higher than that in the epithelial cells at the surrounding crypts, and t

SUBMITTER: Takeda H 

PROVIDER: S-EPMC5705649 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature