Influenza virus-like particles harboring H9N2 HA and NA proteins induce a protective immune response in chicken.

ABSTRACT:

Background

Avian influenza viruses represent a growing threat of an influenza pandemic. The co-circulation of multiple H9N2 genotypes over the past decade has been replaced by one predominant genotype-G57 genotype, which displays a changed antigenicity and improved adaptability in chickens. Effective H9N2 subtype avian influenza virus vaccines for poultry are urgently needed.

Objective

In this study, we constructed H9N2 subtype avian influenza virus-like particle (VLP) and evaluated its protective efficacy in specific pathogen-free (SPF) chickens to lay the foundation for developing an effective vaccine against influenza viruses.

Methods

Expression of influenza proteins in VLPs was confirmed by Western blot, hemagglutination inhibition (HI), and neuraminidase inhibiti

SUBMITTER: Li X 

PROVIDER: S-EPMC5705689 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature