A quantitative method for microstructural analysis of myelinated axons in the injured rodent brain.

ABSTRACT: MRI studies (e.g. using diffusion tensor imaging) revealed that injury to white matter tracts, as observed in for instance perinatal white matter injury and multiple sclerosis, leads to compromised microstructure of myelinated axonal tracts. Alterations in white matter microstructure are also present in a wide range of neurological disorders including autism-spectrum disorders, schizophrenia and ADHD. Whereas currently myelin quantity measures are often used in translational animal models of white matter disease, it can be an important valuable addition to study the microstructural organization of myelination patterns in greater detail. Here, we describe methods to extensively study the microstructure of cortical myelination by immunostaining for myelin. To validate these methods, we caref

SUBMITTER: van Tilborg E 

PROVIDER: S-EPMC5705703 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature