Two Phosphoglucomutase Paralogs Facilitate Ionophore-Triggered Secretion of the Toxoplasma Micronemes.

ABSTRACT: Paralogs of the widely prevalent phosphoglucomutase (PGM) protein called parafusin function in calcium (Ca²⁺)-mediated exocytosis across eukaryotes. In Toxoplasma gondii, the parafusin-related protein 1 (PRP1) has been associated with Ca²⁺-dependent microneme organelle secretion required for essential processes like host cell invasion and egress. Using reverse genetics, we observed PRP1 to be dispensable for completion of the lytic cycle, including host cell invasion and egress by the parasite. However, the absence of the gene affected increased microneme release triggered by A23187, a Ca²⁺ ionophore used to raise the cytoplasmic Ca²⁺ concentration mimicking the physiological role of Ca²⁺ during invasion and egress. The basal le

SUBMITTER: Saha S 

PROVIDER: S-EPMC5705807 | biostudies-literature | 2017 Nov-Dec

REPOSITORIES: biostudies-literature