Investigation of Non-Covalent Interactions of Aflatoxins (B1, B2, G1, G2, and M1) with Serum Albumin.

ABSTRACT: Aflatoxins are widely spread mycotoxins produced mainly by Aspergillus species. Consumption of aflatoxin-contaminated foods and drinks causes serious health risks for people worldwide. It is well-known that the reactive epoxide metabolite of aflatoxin B1 (AFB1) forms covalent adducts with serum albumin. However, non-covalent interactions of aflatoxins with human serum albumin (HSA) are poorly characterized. Thus, in this study the complex formation of aflatoxins was examined with HSA applying spectroscopic and molecular modelling studies. Our results demonstrate that aflatoxins form stable complexes with HSA as reflected by binding constants between 2.1 × 10⁴ and 4.5 × 10⁴ dm³/mol. A binding free energy value of -26.90 kJ mol^-1 suggests a spontaneous binding process betwe

SUBMITTER: Poor M 

PROVIDER: S-EPMC5705954 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature