Arginase 1 deletion in myeloid cells affects the inflammatory response in allergic asthma, but not lung mechanics, in female mice.

ABSTRACT:

Background

(Over-)expression of arginase may limit local availability of arginine for nitric oxide synthesis. We investigated the significance of arginase1 (ARG1) for the development of airway hyperresponsiveness (AHR) and lung inflammation in female mice with ovalbumin (OVA)-induced allergic asthma.

Methods

Arg1 was ablated in the lung by crossing Arg1 ^fl/fl and Tie2Cre ^tg/- mice. OVA sensitization and challenge were conducted, and AHR to methacholine was determined using the Flexivent system. Changes in gene expression, chemokine and cytokine secretion, plasma IgE, and lung histology were quantified using RT-qPCR, ELISA, and immunohistochemistry, respectively.

Results

Arg1 ablation had no influence on the development of OVA-induced AHR, but att

SUBMITTER: Cloots RHE 

PROVIDER: S-EPMC5706166 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature