Optimizing and evaluating the reconstruction of Metagenome-assembled microbial genomes.

ABSTRACT:

Background

Microbiome/host interactions describe characteristics that affect the host's health. Shotgun metagenomics includes sequencing a random subset of the microbiome to analyze its taxonomic and metabolic potential. Reconstruction of DNA fragments into genomes from metagenomes (called metagenome-assembled genomes) assigns unknown fragments to taxa/function and facilitates discovery of novel organisms. Genome reconstruction incorporates sequence assembly and sorting of assembled sequences into bins, characteristic of a genome. However, the microbial community composition, including taxonomic and phylogenetic diversity may influence genome reconstruction. We determine the optimal reconstruction method for four microbiome projects that had variable sequencing platforms (IonTorren

SUBMITTER: Papudeshi B 

PROVIDER: S-EPMC5706307 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature