RNA sequencing reveals candidate genes and polymorphisms related to sperm DNA integrity in testis tissue from boars.

ABSTRACT:

Background

Sperm DNA is protected against fragmentation by a high degree of chromatin packaging. It has been demonstrated that proper chromatin packaging is important for boar fertility outcome. However, little is known about the molecular mechanisms underlying differences in sperm DNA fragmentation. Knowledge of sequence variation influencing this sperm parameter could be beneficial in selecting the best artificial insemination (AI) boars for commercial production. The aim of this study was to identify genes differentially expressed in testis tissue of Norwegian Landrace and Duroc boars, with high and low sperm DNA fragmentation index (DFI), using transcriptome sequencing.

Results

Altogether, 308 and 374 genes were found to display significant differences in expression leve

SUBMITTER: van Son M 

PROVIDER: S-EPMC5706377 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature