A Liquid-Handling Robot for Automated Attachment of Biomolecules to Microbeads.

ABSTRACT: Diagnostics, drug delivery, and other biomedical industries rely on cross-linking ligands to microbead surfaces. Microbead functionalization requires multiple steps of liquid exchange, incubation, and mixing, which are laborious and time intensive. Although automated systems exist, they are expensive and cumbersome, limiting their routine use in biomedical laboratories. We present a small, bench-top robotic system that automates microparticle functionalization and streamlines sample preparation. The robot uses a programmable microcontroller to regulate liquid exchange, incubation, and mixing functions. Filters with a pore diameter smaller than the minimum bead diameter are used to prevent bead loss during liquid exchange. The robot uses three liquid reagents and processes up to 10(7) micro

SUBMITTER: Enten A 

PROVIDER: S-EPMC5706444 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature