Persistent glucose transporter expression on pancreatic beta cells from longstanding type 1 diabetic individuals.

ABSTRACT:

Background

Recent reports have established the notion that many patients with longstanding type 1 diabetes (T1D) possess a remnant population of insulin-producing beta cells. It remains questionable, however, whether these surviving cells can physiologically sense and respond to glucose stimuli.

Methods

Frozen pancreatic sections from non-diabetic donors (n=8), type 2 diabetic patients (n=4), islet autoantibody-positive non-diabetic patients (n=3), type 1 diabetic patients (n=10) and one case of gestational diabetes were obtained via the network for Pancreatic Organ Donors. All longstanding T1D samples were selected based on the detection of insulin-producing beta cells in the pancreas by immunohistochemistry. RNA was isolated from all sections followed by cDNA preparation a

SUBMITTER: Coppieters KT 

PROVIDER: S-EPMC5706467 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature