In vitro enteroid-derived three-dimensional tissue model of human small intestinal epithelium with innate immune responses.

ABSTRACT: There is a need for functional in vitro 3D human intestine systems that can bridge the gap between conventional cell culture studies and human trials. The successful engineering in vitro of human intestinal tissues relies on the use of the appropriate cell sources, biomimetic scaffolds, and 3D culture conditions to support vital organ functions. We previously established a compartmentalized scaffold consisting of a hollow space within a porous bulk matrix, in which a functional and physiologically relevant intestinal epithelium system was generated using intestinal cell lines. In this study, we adopt the 3D scaffold system for the cultivation of stem cell-derived human small intestinal enteriods (HIEs) to engineer an in vitro 3D model of a nonstransformed human small intestinal epithelium.

SUBMITTER: Chen Y 

PROVIDER: S-EPMC5706668 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature