Development of a GAL4-VP16/UAS trans-activation system for tissue specific expression in Medicago truncatula.

ABSTRACT: Promoters with tissue-specific activity are very useful to address cell-autonomous and non cell autonomous functions of candidate genes. Although this strategy is widely used in Arabidopsis thaliana, its use to study tissue-specific regulation of root symbiotic interactions in legumes has only started recently. Moreover, using tissue specific promoter activity to drive a GAL4-VP16 chimeric transcription factor that can bind short upstream activation sequences (UAS) is an efficient way to target and enhance the expression of any gene of interest. Here, we developed a collection of promoters with different root cell layers specific activities in Medicago truncatula and tested their abilities to drive the expression of a chimeric GAL4-VP16 transcription factor in a trans-activation UAS: β-Glu

SUBMITTER: Sevin-Pujol A 

PROVIDER: S-EPMC5706680 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature