Plasma-derived extracellular vesicle proteins as a source of biomarkers for lung adenocarcinoma.

ABSTRACT: Exosomes and other extracellular vesicles (EVs) have been implicated as mediators of intercellular communication. Their release into the circulation has the potential to inform about tumor status. In-depth proteomic characterization of plasma-derived EVs has been limited by challenges in isolating EVs from protein-abundant biological fluids. We implemented a novel single-step density gradient flotation workflow for efficient and rapid isolation of highly enriched circulating EVs from plasma. Mass-spectrometry analysis of plasma EVs from subjects with lung adenocarcinoma and matched controls resulted in the identification of 640 proteins. A total of 108 proteins exhibited significant (p<0.05) differential expression in vesicle preparations derived from lung adenocarcinoma case plasma

SUBMITTER: Vykoukal J 

PROVIDER: S-EPMC5707035 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature