TRPM8 is required for survival and radioresistance of glioblastoma cells.

ABSTRACT: TRPM8 is a Ca²⁺-permeable nonselective cation channel belonging to the melastatin sub-group of the transient receptor potential (TRP) family. TRPM8 is aberrantly overexpressed in a variety of tumor entities including glioblastoma multiforme where it reportedly contributes to tumor invasion. The present study aimed to disclose further functions of TRPM8 in glioma biology in particular upon cell injury by ionizing radiation. To this end, TCGA data base was queried to expose the TRPM8 mRNA abundance in human glioblastoma specimens and immunoblotting was performed to analyze the TRPM8 protein abundance in primary cultures of human glioblastoma. Moreover, human glioblastoma cell lines were irradiated with 6 MV photons and TRPM8 channels were targeted pharmacologically or by RNA inter

SUBMITTER: Klumpp D 

PROVIDER: S-EPMC5707069 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature