Haplotype Counting for Sensitive Chimerism Testing: Potential for Early Leukemia Relapse Detection.

ABSTRACT: Fields of forensics, transplantation, and paternity rely on human identity testing. Currently, this is accomplished through amplification of microsatellites followed by capillary electrophoresis. An alternative and theoretically better approach uses multiple single-nucleotide polymorphisms located within a small region of DNA, a method we initially developed using HLA-A and called haplotype counting. Herein, we validated seven additional polymorphic loci, sequenced a total of 45 individuals from three of the 1000 Genomes populations (15 from each), and determined the number of haplotypes, heterozygosity, and polymorphic information content for each locus. In addition, we developed a multiplex PCR that amplifies five of these loci simultaneously. Using this strategy with a small cohort of l

SUBMITTER: Debeljak M 

PROVIDER: S-EPMC5707182 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature