Charge-state Resolved Infrared Multiple Photon Dissociation (IRMPD) Spectroscopy of Ubiquitin Ions in the Gas Phase.

ABSTRACT: In this study, we obtained for the first time the direct infrared multiple photon dissociation (IRMPD) spectra of ubiquitin ions in the range 2700-3750 cm^-1. Ubiquitin ions with different charge states showed absorption in the two regions of 2940-3000 cm^-1 and 3280-3400 cm^-1. The increase of the charge state of ubiquitin ions broadened the absorption peak on the high-frequency side in the second region, indicating some hydrogen bonds were weakened due to Coulomb interaction. It is also found that the relative intensity of the absorption peak in the first region compared to the absorption peak in the second region increased with increasing charge state, making the IRMPD spectra charge-state resolved. Although it is usually reasonable to suggest the origin of

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5707388 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature