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Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes.

ABSTRACT: Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by probe JG45.

SUBMITTER: Diaz de Grenu B

PROVIDER: S-EPMC5707467 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes.

Díaz de Greñu Borja B García-Calvo José J Cuevas José J García-Herbosa Gabriel G García Begoña B Busto Natalia N Ibeas Saturnino S Torroba Tomás T Torroba Blanca B Herrera Antonio A Pons Sebastian S

Chemical science 20150430 7

Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+< ...[more]

PMID: 29218145

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