Micro-competition system for Raman quantification of multiple glycans on intact cell surface.

ABSTRACT: A micro-competition system is designed for simultaneous quantification of multiple glycans on intact cell surfaces, by integrating two-surface-one-molecule competition with surface enhanced Raman scattering (SERS). The micro-competition is achieved among multiple-polysaccharide-coated gold nanostars functionalized silica bubbles, target cells and gold nanoprobes at a micron scale. The gold nanoprobes are prepared by coating distinct Raman molecules and lectins on gold nanoparticles for signal resolution and glycan recognition, respectively. The silica bubble surface serves as an artificial glycan surface and a SERS substrate. Upon the competitive recognition of lectin to the corresponding glycan, the gold nanoprobes can be specifically captured by the bubbles and cells in a homogeneous sys

SUBMITTER: Chen Y 

PROVIDER: S-EPMC5707490 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature