Target-driven DNA association to initiate cyclic assembly of hairpins for biosensing and logic gate operation.

ABSTRACT: A target-driven DNA association was designed to initiate cyclic assembly of hairpins, which led to an enzyme-free amplification strategy for detection of a nucleic acid or aptamer substrate and flexible construction of logic gates. The cyclic system contained two ssDNA (S1 and S2) and two hairpins (H1 and H2). These ssDNA could co-recognize the target to produce an S1-target-S2 structure, which brought their toehold and branch-migration domains into close proximity to initiate the cyclic assembly of hairpins. The assembly product further induced the dissociation of a double-stranded probe DNA (Q:F) via toehold-mediated strand displacement to switch the fluorescence signal. This method could detect DNA and ATP as model analytes down to 21.6 pM and 38 nM, respectively. By designing di

SUBMITTER: Guo Y 

PROVIDER: S-EPMC5707516 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature