Begomoviral Movement Protein Effects in Human and Plant Cells: Towards New Potential Interaction Partners.

ABSTRACT: Geminiviral single-stranded circular DNA genomes replicate in nuclei so that the progeny DNA has to cross both the nuclear envelope and the plasmodesmata for systemic spread within plant tissues. For intra- and intercellular transport, two proteins are required: a nuclear shuttle protein (NSP) and a movement protein (MP). New characteristics of ectopically produced Abutilon mosaic virus (AbMV) MP (MP^AbMV), either authentically expressed or fused to a yellow fluorescent protein or epitope tags, respectively, were determined by localization studies in mammalian cell lines in comparison to plant cells. Wild-type MP^AbMV and the distinct MP^AbMV: reporter protein fusions appeared as curled threads throughout mammalian cells. Co-staining with cytoskeleton markers

SUBMITTER: Krapp S 

PROVIDER: S-EPMC5707541 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature