An open-source method to analyze optokinetic reflex responses in larval zebrafish.

ABSTRACT:

Background

Optokinetic reflex (OKR) responses provide a convenient means to evaluate oculomotor, integrative and afferent visual function in larval zebrafish models, which are commonly used to elucidate molecular mechanisms underlying development, disease and repair of the vertebrate nervous system.

New method

We developed an open-source MATLAB-based solution for automated quantitative analysis of OKR responses in larval zebrafish. The package includes applications to: (i) generate sinusoidally-transformed animated grating patterns suitable for projection onto a cylindrical screen to elicit the OKR; (ii) determine and record the angular orientations of the eyes in each frame of a video recording showing the OKR response; and (iii) analyze angular orientation data from the tr

SUBMITTER: Scheetz SD 

PROVIDER: S-EPMC5708171 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature