Lateral flow assay for carbohydrate antigen 19-9 in whole blood by using magnetized carbon nanotubes.

ABSTRACT: The authors describe a rapid, low-cost and sensitive approach for the determination of carbohydrate antigen 19-9 (CA 19-9) in whole blood by using magnetized carbon nanotube (MCNT) and lateral flow strip biosensor (LFSB). MCNTs were synthesized by depositing magnetite (Fe₃O₄) nanoparticles on multiwalled carbon nanotube (CNT) via co-precipitation of ferric and ferrous ions within a dispersion of shortened multiwalled CNTs. Antibody against CA 19-9 (Ab₁) was covalently immobilized on the MCNTs and were used to capture CA 19-9 in blood. After magnetic separation, the formed MCNT-Ab₁-CA 19-9 complexes are applied to the LFSB, in which a capture antibody (Ab₂) and a secondary antibody (Ab₃) are immobilized on the test zone and c

SUBMITTER: Huang Y 

PROVIDER: S-EPMC5708549 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature