Improved Detection of Cytokines Produced by Invariant NKT Cells.

ABSTRACT: Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity.

SUBMITTER: Sag D 

PROVIDER: S-EPMC5709402 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature