Raman-Deuterium Isotope Probing for in-situ identification of antimicrobial resistant bacteria in Thames River.

ABSTRACT: The emergence and widespread distribution of antimicrobial resistant (AMR) bacteria has led to an increasing concern with respect to potential environmental and public health risks. Culture-independent and rapid identification of AMR bacteria in-situ in complex environments is important in understanding the role of viable but non-culturable and antibiotic persistent bacteria and in revealing potential pathogens without waiting for colony formation. In this study, a culture-independent and non-destructive phenotyping approach, so called Raman Deuterium Stable Isotope Probing (Raman-DIP), was developed to identify AMR bacteria in the River Thames. It is demonstrated that Raman-DIP was able to accurately identify resistant and susceptible bacteria within 24 hours. The work shows that, in the

SUBMITTER: Song Y 

PROVIDER: S-EPMC5709456 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature