Isoform-specific localization of DNMT3A regulates DNA methylation fidelity at bivalent CpG islands.

ABSTRACT: DNA methylation is a prevalent epigenetic modification involved in transcriptional regulation and essential for mammalian development. While the genome-wide distribution of this mark has been studied to great detail, the mechanisms responsible for its correct deposition, as well as the cause for its aberrant localization in cancers, have not been fully elucidated. Here, we have compared the activity of individual DNMT3A isoforms in mouse embryonic stem and neuronal progenitor cells and report that these isoforms differ in their genomic binding and DNA methylation activity at regulatory sites. We identify that the longer isoform DNMT3A1 preferentially localizes to the methylated shores of bivalent CpG island promoters in a tissue-specific manner. The isoform-specific targeting of DNMT3A1 co

SUBMITTER: Manzo M 

PROVIDER: S-EPMC5709737 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature