Molecular basis for inner kinetochore configuration through RWD domain-peptide interactions.

ABSTRACT: Kinetochores are dynamic cellular structures that connect chromosomes to microtubules. They form from multi-protein assemblies that are evolutionarily conserved between yeasts and humans. One of these assemblies-COMA-consists of subunits Ame1^CENP-U, Ctf19^CENP-P, Mcm21^CENP-O and Okp1^CENP-Q A description of COMA molecular organization has so far been missing. We defined the subunit topology of COMA, bound with inner kinetochore proteins Nkp1 and Nkp2, from the yeast Kluyveromyces lactis, with nanoflow electrospray ionization mass spectrometry, and mapped intermolecular contacts with hydrogen-deuterium exchange coupled to mass spectrometry. Our data suggest that the essential Okp1 subunit is a multi-segmented nexus with distinct binding sites

SUBMITTER: Schmitzberger F 

PROVIDER: S-EPMC5709738 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature