Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis.

ABSTRACT:

Background

Adipokines are bioactive hormones secreted by adipose tissues. Resistin, an adipokine, plays important roles in the regulation of insulin resistance and inflammation. Resistin levels are known to be increased in the serum and synovial fluid of rheumatoid arthritis (RA) patients. However, the pathogenic role of resistin in RA has not yet been elucidated.

Methods

The expression of resistin and adenylate cyclase-associated protein 1 (CAP1), a receptor for resistin, was examined immunohistochemically in synovial tissue. CAP1 expression in in vitro cultured fibroblast-like synoviocytes (FLSs) was assessed with a reverse transcription-polymerase chain reaction (PCR) and western blotting. The gene expression of resistin-stimulated FLSs was evaluated by RNA sequencing (RN

SUBMITTER: Sato H 

PROVIDER: S-EPMC5709830 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature