Functional dissection of Drosophila melanogaster SUUR protein influence on H3K27me3 profile.

ABSTRACT:

Background

In eukaryotes, heterochromatin replicates late in S phase of the cell cycle and contains specific covalent modifications of histones. SuUR mutation found in Drosophila makes heterochromatin replicate earlier than in wild type and reduces the level of repressive histone modifications. SUUR protein was shown to be associated with moving replication forks, apparently through the interaction with PCNA. The biological process underlying the effects of SUUR on replication and composition of heterochromatin remains unknown.

Results

Here we performed a functional dissection of SUUR protein effects on H3K27me3 level. Using hidden Markow model-based algorithm we revealed SuUR-sensitive chromosomal regions that demonstrated unusual characteristics: They do not contain Polyco

SUBMITTER: Posukh OV 

PROVIDER: S-EPMC5709859 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature